Gel Electrophoresis - 679 Words

General Biology and Lab II - BIO196 Gel electrophoresis Report Sheet Name: Date: Partner: Conlusions The results of my testing indicates that: Blood from Suspect Number __________ was at the crime scene. Explain your evidence for coming to this conclusion. Is this sufficient evidence to convict the the suspect of the crime? Why or why not? The DNA found on the crime scene matches the one from suspect 3 through same number of cuts and size of the banded fragments which are both identical. This shows that two samples of DNA have identical restriction sites that produced identical patterns. When also calculating the number of base pairs of all samples, we find that there is a 97.76% match with Suspect 3s DNA with the crime scene DNA†¦show more content†¦11 125 500 12 132 400 13 138 300 Name: Date: DNA Fragment Size Standard Curve You do not need to complete this graph if you attach the printout of the computer generated graph 100,000 50,000 23,130 10,000 Fragment Size (base pairs - bp) 8000 6,000 5,000 4,000 3,000 2,000 1,500 1,400 1,000 750 500 400 300 200 100 0 5 10 15 20 25 30 35 Distance Traveled (mm) Name:Show MoreRelatedWhat Are Gel Electrophoresis?1157 Words   |  5 PagesGel electrophoresis is a gel technique that separates DNA and proteins based on their mass, by means of an applied electrical field that passes through an agarose or polyacrylamide gel. The concentration of agarose in the gel is commonly 0.8% to 1.0%, since agarose is expensive. The gel is embedded on a buffer, pH of 8.3, which keeps the pH of the solution at equilibrium. Assuming that at typical pH, DNA is negatively charged, denatured protein samples are placed in the wells located on the negativeRead MoreCrime Scenes: Agarose Gel Electrophoresis Essay1467 Words   |  6 PagesDNA. Most of the time, this is done using a technique known as gel electrophoresis. Gel electrophoresis is a method used to separate the macromolecules that make up nucleic acids, such as DNA and RNA, along with proteins. Gel electrophoresis is significant because it has given scientists insight on what cell s cause certain diseases and has led to advancements in DNA and fingerprint identification. My experiment will use gel electrophoresis to compare the separation of food dye in different agarose concentrationsRead MoreThe Use Of Sodium Dodecyl Sulphate ( Sds ) Polyacrylamide Gel Electrophoresis Essay1718 Words   |  7 Pagesreferences, expanded on experimental errors in more detail and ensured my aim and conclusion is clear. Abstract The purpose of this investigation was to identify the class of immunoglobulins using, sodium dodecyl sulphate (SDS) Polyacrylamide Gel Electrophoresis (PAGE); by making deductions about the structure and molecular organisation of the protein. The experiment was conducted to calculate the unknown molecular mass for reduced and non-reduced immunoglobulin, using SDS-PAGE by measuring the distanceRead MoreThe Extraction Of Dna From A Kiwi Fruit Run Through Agarose Gel Electrophoresis1383 Words   |  6 PagesTitle: The Extraction of DNA from a Kiwi Fruit Run Through Agarose Gel Electrophoresis. Introduction: In this experiment the use of gel electrophoresis and DNA extraction will be used to see that kiwi fruit has evidence of DNA. The aim is to be able to extract the DNA from a Kiwi Fruit then be able to load then visualize the DNA that was extracted through the use of Gel Electrophoresis. Materials: The materials used were Kiwi Fruit (peeled and cut into triangles), Plastic zip lock bag, I – 10 mLRead MoreGel Electrophoresis: Separating DNA and RNA848 Words   |  4 Pages Gel electrophoresis is a procedure used in laboratories to separate DNA, as well as RNA and proteins. A gel slab is placed in a buffer-filled box and an electrical field is applied. The negatively charged DNA will migrate towards the positively charged side, where it can then be recorded and further analyzed. An example for the use of gel electrophoresis would be in identifying people. DNA is present in almost every cell of our body. Each person has a unique sequence of DNA base pairs that makesRead MoreA Study On A Gel Electrophoresis Experiment1016 Words   |  5 Pagesstill be 0.48. 3. A. A gel electrophoresis experiment works because DNA has negative charge and is attracted to the positive electrode. The different mass of DNA segments with different lengths separates them as they move across the gel. Shorter DNA segments move further toward the end of the gel while longer DNA segments lag behind. This experiment is set up by preparing for a gel with agar solution of a certain concentration and putting two electrodes at its two ends. The gel in the question wouldRead MorePcr Lab Analyzing The Alu Pv92 Genetic Sequence1607 Words   |  7 Pagesconsisted of 26 individuals, of varying ethnical backgrounds, ages, and genders, who amplified their own DNA through the use of PCR. Their DNA was examined for the presence [(+/+) or (+/-)] or absence [(-/-)] of the Alu insertion through the use of gel electrophoresis. Based on the hypothesis, the objective of this experiment is to prepare and perform a polymerase chain reaction to determine the amount of students between the two lab sections that present at least one copy of the Alu allele in the genomeRead MoreWestern Blotting Essay1559 Words   |  7 Pagesand is used to distinguish from different species based on variation, commonality, or evolutionary divergence. First, proteins are extracted from the tissue and loaded into a gel matrix. The matrix will separate the proteins according to size using an electric current. Proteins that are separated after are blotted from the gel and onto a paper membrane. An antibody is then added to the membrane paper and causes a colored reaction. Following the reaction, the results help detect and quantify a singleRead MoreThe Isolation Of Milk Whey904 Words   |  4 Pagesusing the *** as the blank. Day 3: Protein Characterization Using SDS-PAGE Gel Electrophoresis and BCA Assay A crude whey sample and a purified *-lactalbumin sample were prepared for SDS-PAGE gel electrophoresis by mixing 20  µL of each sample with  µL of the reducing gel buffer in Eppendorf tubes. These two samples were then boiled for 5 minutes and then allowed to cool to room temperature. A precast gel was inserted into the gel running apparatus and the comb was removed. Consequently, a 10x stock solutionRead Morecomparative proteomics Essay944 Words   |  4 Pages METHODS The method used in this lab to map the proteins was the method of Polyacrylamide gel electrophoresis. This method can be used to separate the proteins present in the fish muscle and separates them on size. Due to the fact that they are separated by size, the proteins can be compared because similar proteins with stop at the same spot in the gel. So measuring the bands that show up on the gel you can determine if different fish species have similar proteins. The first thing that is